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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-62944 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLHL11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NSDDIDKQYR KEAYRYCAER KRWMLLPPMP QPRCRATACH VRIPYRYLHG TQRYPMPQNL MWQKDRIRQM QEIHRHALNM RRVPSSQIEC
- Concentration
- 0.1 mg/mL
Submitted references Immunopathogenesis and proposed clinical score for identifying Kelch-like protein-11 encephalitis.
Vogrig A, Péricart S, Pinto AL, Rogemond V, Muñiz-Castrillo S, Picard G, Selton M, Mittelbronn M, Lanoiselée HM, Michenet P, Benaiteau M, Pariente J, Zéphir H, Giordana C, Montaut S, Salhi H, Bachoumas P, Montcuquet A, Letovanec I, Uro-Coste E, Honnorat J
Brain communications 2021;3(3):fcab185
Brain communications 2021;3(3):fcab185
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLHL11 in control (vector only transfected HEK293T lysate) and KLHL11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a KLHL11 Polyclonal Antibody (Product # PA5-62944).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KLHL11 in human nasopharynx shows distinct membranous positivity in respiratory epithelial cells. Samples were probed using a KLHL11 Polyclonal Antibody (Product # PA5-62944).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Immunostaining pattern in patients with KLHL11-Abs. Reactivity of rat brain tissue using a negative control (row 1) and with patient CSF positive for KLHL11-Abs (rows 2, 3 and 4). The CSF of patients showed extensive and diffuse immunostaining involving the cerebellar cortex and nuclei, putamen and hippocampus (in particular, the CA3 region, with a 'comb-like' staining). In the cerebellum and basal ganglia the immunostaining showed a 'leopard-like' appearance. For comparison, immunostaining using a commercial KLHL11-Abs is also shown (row 5), highlighting the involvement of the same regions.
