MAB7467

antibody from Novus Biologicals

Targeting: S100A6 2A9, CABP, CACY, PRA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7467

Provider

Novus Biologicals

Product name

Mouse Monoclonal S100A6 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human S100A6 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse S100A6 and no cross-reactivity with recombinant human (rh) S100A1, rhS100A4, or rhS100B is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse S100A6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A6 Monoclonal Antibody (Catalog # MAB7467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A6 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

S100A6 in Human Kidney. S100A6 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human S100A6 Monoclonal Antibody (Catalog # MAB7467) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to glomeruli and distal tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.