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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-80989 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-S100A6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human S100A6.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A6 in Lane A: Hela Whole Cell Lysate (30 µg), Lane B: A549 Whole Cell Lysate (30 µg) . Samples were probed using a S100A6 Polyclonal Antibody (Product # PA5-80989) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:10 kDa. Observed band size:10 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human S100A6 in human renal carcinoma (from 3 donors) with S100A6 Polyclonal Antibody (Product # PA5-80989) (1:10000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human S100A6 in human lung cancer with S100A6 Polyclonal Antibody (Product # PA5-80989) (1:10000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human S100A6 in human hepatoma (from 2 donors) with S100A6 Polyclonal Antibody (Product # PA5-80989) (1:10000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CK14 in rat skin with S100A6 Polyclonal Antibody (Product # PA5-80989) (1:1000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- S100A6 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg A549 Whole Cell Lysate, 2 µL of S100A6 Polyclonal Antibody and 15 µL of 50% Protein G agarose. Primary Antibody: S100A6 Polyclonal Antibody (Product # PA5-80989), at 1:200 dilution. Secondary Antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Immunoprecipitation was performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 12 kDa.
