HPA007575

S100A6 antibody from Atlas Antibodies

2A9, CABP, CACY, PRA

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

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Antibody Data

Product number

HPA007575

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA007575, RRID:AB_888796

Product name

Anti-S100A6

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AIFHKYSGREGDKHTLSKKELKELIQKELTIGSKL
QDAEIARLMEDLDRNKDQEVNFQEYVTFLGALALI
YNE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Chronic acidosis in the tumour microenvironment selects for overexpression of LAMP2 in the plasma membrane
Damaghi M, Tafreshi N, Lloyd M, Sprung R, Estrella V, Wojtkowiak J, Morse D, Koomen J, Bui M, Gatenby R, Gillies R
Nature Communications 2015 December;6

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line CAPAN-2.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Sample type

MOUSE, RAT

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines PC-3 and HEK293 using Anti-S100A6 antibody. Corresponding S100A6 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human urinary bladder and liver tissues using Anti-S100A6 antibody. Corresponding S100A6 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more