39-6100

antibody from Invitrogen Antibodies

Targeting: TACR1 NK1R, NKIR, SPR, TAC1R

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

39-6100

Provider

Invitrogen Antibodies

Product name

TACR1 Monoclonal Antibody (ZN003)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

ZN003

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Respiratory disturbances in a mouse model of Parkinson's disease.

Oliveira LM, Oliveira MA, Moriya HT, Moreira TS, Takakura AC
Experimental physiology 2019 May;104(5):729-739

---

Human microglia and astrocytes constitutively express the neurokinin-1 receptor and functionally respond to substance P.

Burmeister AR, Johnson MB, Chauhan VS, Moerdyk-Schauwecker MJ, Young AD, Cooley ID, Martinez AN, Ramesh G, Philipp MT, Marriott I
Journal of neuroinflammation 2017 Dec 13;14(1):245

---

Cyclophosphamide causes activation of protein kinase A (PKA) in the brainstem of vomiting least shrews (Cryptotis parva).

Alkam T, Chebolu S, Darmani NA
European journal of pharmacology 2014 Jan 5;722:156-64

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Neurokinin 1 Receptor / NK1R Receptor was performed using 90% confluent log phase Neuro 2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurokinin 1 Receptor / NK1R (ZN003) Mouse Monoclonal Antibody (Product # 39-6100) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Neurokinin 1 Receptor / NK1R was done on Neuro-2a cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Neurokinin 1 Receptor / NK1R Mouse Monoclonal Antibody (Product # 39-6100, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..