AP09291PU-N

antibody from Acris Antibodies GmbH

Targeting: SLIT3 MEGF5, SLIL2, Slit-3, SLIT1, slit2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09291PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09291PU-N, RRID:AB_2035961

Product name

anti SLIT3 (1164-1177)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding aa 1164-1177 of Human SLIT-3 protein

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.83 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35 µg of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results