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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX129235 - Provider product page
- Provider
- GeneTex
- Product name
- SOX3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX3 antibody detects SOX3 protein by western blot analysis.A. 30 £gg MCF-7 whole cell lysate/extract10 % SDS-PAGESOX3 antibody (GTX129235) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX3 antibody detects SOX3 protein at nucleus by immunofluorescent analysis.Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX3 protein stained by SOX3 antibody (GTX129235) diluted at 1:500.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SOX3 protein from NT2D1 whole cell extracts using 5 £gg of SOX3 antibody (GTX129235).Western blot analysis was performed using SOX3 antibody (GTX129235).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX3 antibody detects SOX3 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SOX3 antibody (GTX129235) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX3 antibody detects SOX3 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SOX3 antibody (GTX129235) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX3 antibody detects SOX3 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: SOX3 antibody (GTX129235) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
