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antibody from Invitrogen Antibodies
Targeting: PRDX6
1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- LF-MA0018 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PRDX6 Monoclonal Antibody (4A3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is HeLa cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4A3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Peroxiredoxin 5 Inhibits Glutamate-Induced Neuronal Cell Death through the Regulation of Calcineurin-Dependent Mitochondrial Dynamics in HT22 Cells.
Ginkgolic Acid Rescues Lens Epithelial Cells from Injury Caused by Redox Regulated-Aberrant Sumoylation Signaling by Reviving Prdx6 and Sp1 Expression and Activities.
Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes.
Kim MH, Lee HJ, Lee SR, Lee HS, Huh JW, Bae YC, Lee DS
Molecular and cellular biology 2019 Oct 15;39(20)
Molecular and cellular biology 2019 Oct 15;39(20)
Ginkgolic Acid Rescues Lens Epithelial Cells from Injury Caused by Redox Regulated-Aberrant Sumoylation Signaling by Reviving Prdx6 and Sp1 Expression and Activities.
Chhunchha B, Singh P, Singh DP, Kubo E
International journal of molecular sciences 2018 Nov 8;19(11)
International journal of molecular sciences 2018 Nov 8;19(11)
Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes.
Daverey A, Agrawal SK
Neuroscience 2016 Oct 1;333:92-103
Neuroscience 2016 Oct 1;333:92-103
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of LNCaP (Lane 1), MCF7 (Lane 2), PC-3 (Lane 3), HeLa (Lane 4), HCT 116 (Lane 5), COLO 205 (Lane 6) and MDA-MB-231 (Lane 7). The blot was probed with Anti-PRDX6 Mouse Monoclonal Antibody (Product # LF-MA0018, 0.5 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 25 kDa band corresponding to PRDX6 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PRDX6 was achieved by transfecting MCF7 cells with PRDX6 specific siRNAs (Silencer® select Product # s18429). Western blot analysis (Fig a) was performed using membrane enriched extracts from the PRDX6 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti- PRDX6 Mouse monoclonal Antibody (Product # LF-MA0018, 1µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:5000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PRDX6.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PRDX6 was done on LNCap cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PRDX6 Mouse Monoclonal Antibody (Product # LF-MA0018, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
