13585-1-AP

antibody from Proteintech Group

Targeting: PRDX6 1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13585-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13585-1-AP, RRID:AB_2168637

Product name

PRDX6 antibody

Antibody type

Polyclonal

Description

KD/KO validated PRDX6 antibody (Cat. #13585-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Central role of Sigma-1 receptor in ochratoxin A-induced ferroptosis.

Chen W, Han L, Yang R, Wang H, Yao S, Deng H, Liu S, Zhou Y, Shen XL
Archives of toxicology 2024 Oct;98(10):3323-3336

---

Glycyrrhizin regulates antioxidation through Nrf2 signaling pathway in rat temporomandibular joint osteoarthritis.

Hu Z, Zhao J, Liu X, Li Y, Jiang H, Fang W, Long X
Journal of oral rehabilitation 2024 Mar;51(3):611-622

---

PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis.

Fujita H, Tanaka YK, Ogata S, Suzuki N, Kuno S, Barayeu U, Akaike T, Ogra Y, Iwai K
Nature structural & molecular biology 2024 Aug;31(8):1277-1285

---

PRDX6 alleviated heart failure by inhibiting doxorubicin-induced ferroptosis through the JAK2/STAT1 pathway inactivation.

Xiong J, Zhou R, Deng X
In vitro cellular & developmental biology. Animal 2024 Apr;60(4):354-364

---

Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis.

Guo Q, Zhao M, Wang Q, Lu T, Luo P, Chen L, Xia F, Pang H, Shen S, Cheng G, Dai C, Meng Y, Zhong T, Qiu C, Wang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Apr;173:116304

---

The PRX-1/TLR4 axis promotes hypoxia-induced radiotherapy resistance in non-small cell lung cancer by targeting the NF-κB/p65 pathway.

Hao J, Song Z, Su J, Li L, Zou L, Zou K
Cellular signalling 2023 Oct;110:110806

---

Peroxiredoxin-6 Released by Astrocytes Contributes to Neuroapoptosis During Ischemia.

Hou JY, Zhou XL, Wang XY, Liang J, Xue Q
Neuroscience 2023 Feb 21;512:59-69

---

Curcumin strengthens a spontaneous self-protective mechanism-SP1/PRDX6 pathway, against di-n-butyl phthalate-induced testicular ferroptosis damage.

Bu H, Wang B, Wu Y, Li P, Cui Y, Jiang X, Yu X, Liu B, Tang M
Environmental science and pollution research international 2023 Dec;30(58):122165-122181

---

Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line.

Bidooki SH, Sánchez-Marco J, Martínez-Beamonte R, Herrero-Continente T, Navarro MA, Rodríguez-Yoldi MJ, Osada J
International journal of molecular sciences 2023 Dec 5;24(24)

---

Construction and validation of a novel ferroptosis-related prognostic signature for lung adenocarcinoma.

Yang L, Fan X, Zhou C, Wang Z, Cui Z, Wu X, Xu Z, Yang J, Zhang X
Translational lung cancer research 2023 Aug 30;12(8):1766-1781

---

Comparative Analysis of Proteomic of Curcumin Reversing Multidrug Resistance in HCT-8/VCR Cells.

Li L, Yu L, Cao X, Zhang C, Liu Q, Chen J
Journal of oncology 2022;2022:3605436

---

PRDX6 alleviates lipopolysaccharide-induced inflammation and ferroptosis in periodontitis.

Yang WY, Meng X, Wang YR, Wang QQ, He X, Sun XY, Cheng N, Zhang L
Acta odontologica Scandinavica 2022 Oct;80(7):535-546

---

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Wang D, Li Y, Liu Y, Cheng S, Liu F, Zuo R, Ding C, Shi S, Liu G
The international journal of biochemistry & cell biology 2022 Jun;147:106233

---

Notch3 regulates ferroptosis via ROS-induced lipid peroxidation in NSCLC cells.

Li Z, Xiao J, Liu M, Cui J, Lian B, Sun Y, Li C
FEBS open bio 2022 Jun;12(6):1197-1205

---

Chronic angiotensin receptor activation promotes hepatic triacylglycerol accumulation during an acute glucose challenge in obese-insulin-resistant OLETF rats.

Godoy-Lugo JA, Thorwald MA, Hui DY, Nishiyama A, Nakano D, Soñanez-Organis JG, Ortiz RM
Endocrine 2022 Jan;75(1):92-107

---

Knocking Down Peroxiredoxin 6 Aggravates Cerebral Ischemia-Reperfusion Injury by Enhancing Mitophagy.

Hong T, Zhou Y, Peng L, Wu X, Li Y, Li Y, Zhao Y
Neuroscience 2022 Feb 1;482:30-42

---

Identification of a Novel Ferroptosis-Related Gene Prognostic Signature in Bladder Cancer.

Sun J, Yue W, You J, Wei X, Huang Y, Ling Z, Hou J
Frontiers in oncology 2021;11:730716

---

Hypothermic oxygenated perfusion with defatting cocktail further improves steatotic liver grafts in a transplantation rat model.

Lin F, Zhen F, Yan X, Shaojun Y, Guizhu P, Yanfeng W, Qifa Y
Artificial organs 2021 Sep;45(9):E304-E316

---

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Huang H, Zhang S, Li Y, Liu Z, Mi L, Cai Y, Wang X, Chen L, Ran H, Xiao D, Li F, Wu J, Li T, Han Q, Chen L, Pan X, Li H, Li T, He K, Li A, Zhang X, Zhou T, Xia Q, Man J
Nature communications 2021 Jun 17;12(1):3720

---

Peroxiredoxins are involved in the pathogenesis of multiple sclerosis and neuromyelitis optica spectrum disorder.

Uzawa A, Mori M, Masuda H, Ohtani R, Uchida T, Aoki R, Kuwabara S
Clinical and experimental immunology 2020 Nov;202(2):239-248

---

Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents.

Xu C, Zhou W, Dong G, Qiao H, Peng J, Jia P, Li Y, Liu H, Sun K, Zhao W
Bioorganic chemistry 2020 Dec;105:104424

---

Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.

Ma Z, Lu Y, Yang F, Li S, He X, Gao Y, Zhang G, Ren E, Wang Y, Kang X
Toxicology and applied pharmacology 2020 Apr 19;397:115014

---

Identification of PRDX6 as a regulator of ferroptosis.

Lu B, Chen XB, Hong YC, Zhu H, He QJ, Yang B, Ying MD, Cao J
Acta pharmacologica Sinica 2019 Oct;40(10):1334-1342

---

The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages.

Gong Y, Rao PSS, Sinha N, Ranjit S, Cory TJ, Kumar S
Biochemistry and biophysics reports 2019 Mar;17:65-70

---

4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals.

Huang S, Cao S, Zhou T, Kong L, Liang G
Environmental toxicology and pharmacology 2018 Sep;62:234-243

---

Luteolin ameliorates rat myocardial ischaemia-reperfusion injury through activation of peroxiredoxin II.

Wei B, Lin Q, Ji YG, Zhao YC, Ding LN, Zhou WJ, Zhang LH, Gao CY, Zhao W
British journal of pharmacology 2018 Aug;175(16):3315-3332

---

Nucleophosmin Regulates Intracellular Oxidative Stress Homeostasis via Antioxidant PRDX6.

Liu GY, Shi JX, Shi SL, Liu F, Rui G, Li X, Gao LB, Deng XL, Li QF
Journal of cellular biochemistry 2017 Dec;118(12):4697-4707

---

Peroxiredoxin 6 Is a Crucial Factor in the Initial Step of Mitochondrial Clearance and Is Upstream of the PINK1-Parkin Pathway.

Ma S, Zhang X, Zheng L, Li Z, Zhao X, Lai W, Shen H, Lv J, Yang G, Wang Q, Ji J
Antioxidants & redox signaling 2016 Mar 20;24(9):486-501

---

Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs.

Sahu N, Stephan JP, Cruz DD, Merchant M, Haley B, Bourgon R, Classon M, Settleman J
Nature communications 2016 Aug 3;7:12351

---

MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism.

Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI
Mitochondrion 2015 Jan;20:43-51

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 13585-1-AP(PRDX6 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PRDX6 antibody 13585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 13585-1-AP(PRDX6 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 13585-1-AP(PRDX6 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue