Explore
Validate
LearnAF3490
antibody from Novus Biologicals
Targeting: PRDX6
1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3490 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Peroxiredoxin 6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse, and rat Peroxiredoxin 6 on in Western blots. In Western blots, this antibody does not cross-react with recombinant human Peroxiredoxin 1, 2, 3, or 5.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation.
Macco R, Pelizzoni I, Consonni A, Vitali I, Giacalone G, Martinelli Boneschi F, Codazzi F, Grohovaz F, Zacchetti D
Journal of neuroinflammation 2013 Oct 28;10:130
Journal of neuroinflammation 2013 Oct 28;10:130
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Peroxiredoxin 6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
