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LearnMA5-29500
antibody from Invitrogen Antibodies
Targeting: PRDX6
1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-29500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRDX6 Monoclonal Antibody (3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 3
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRDX6 in Lane A: Hela Whole Cell Lysate (30 µg), Lane B: 293T Whole Cell Lysate (30 µg), Lane C: Jurkat Whole Cell Lysate (30 µg), Lane D: MCF7 Whole Cell Lysate (30 µg). Samples were probed using a PRDX6 Monoclonal Antibody (Product # MA5-29500) at a 1:2000 dilution, followed by a Goat Anti-Mouse IgG (H+L), Dylight 800 Secondary Antibody at a 1:150000 dilution. Western blot was performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRDX6 Monoclonal Antibody (3) (Product # MA5-29500) at 1:2,000 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 27 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRDX6 Monoclonal Antibody (3) (Product # MA5-29500) at 1:2,000 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 27 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human PRDX6 in human brain with PRDX6 Monoclonal Antibody (3) (Product # MA5-29500, 1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to neuroglia cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRDX6 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 µL with PRDX6 Monoclonal Antibody (3) (Product # MA5-29500) and 15 µL of 50 % Protein G agarose. Primary antibody: PRDX6 Monoclonal Antibody (3), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.
