MA5-29500

antibody from Invitrogen Antibodies

Targeting: PRDX6 1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29500

Provider

Invitrogen Antibodies

Product name

PRDX6 Monoclonal Antibody (03)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human Peroxiredoxin 6/PRDX6.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRDX6 in Lane A: Hela Whole Cell Lysate (30 µg), Lane B: 293T Whole Cell Lysate (30 µg), Lane C: Jurkat Whole Cell Lysate (30 µg), Lane D: MCF7 Whole Cell Lysate (30 µg). Samples were probed using a PRDX6 Monoclonal Antibody (Product # MA5-29500) at a 1:2000 dilution, followed by a Goat Anti-Mouse IgG (H+L), Dylight 800 Secondary Antibody at a 1:150000 dilution. Western blot was performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PRDX6 Monoclonal Antibody (3) (Product # MA5-29500) at 1:2,000 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 27 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PRDX6 Monoclonal Antibody (3) (Product # MA5-29500) at 1:2,000 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 27 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PRDX6 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 µL with PRDX6 Monoclonal Antibody (3) (Product # MA5-29500) and 15 µL of 50 % Protein G agarose. Primary antibody: PRDX6 Monoclonal Antibody (3), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.