MA5-34906
antibody from Invitrogen Antibodies
Targeting: PRDX6
1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-34906 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRDX6 Monoclonal Antibody (7G1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: PC-3M, K562, A431, SiHa, human liver tissue, human colon cancer tissue, human kidney tissue, human placenta tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7G1
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRDX6 in PC-3M (1) and K562 (2). Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Peroxiredoxin-6 was performed by loading 25 µg of WT (lane 1) and PRDX6 CRISPR KO (lane 2) HAP1 cell lysates in RIPA buffer onto a 8-16% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. PRDX6 was detected at approximately 25 kDa (designated by the black arrow) using a PRDX6 monoclonal antibody (Product # MA5-34906) at a dilution of 1:500 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 62-6520) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRDX6 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRDX6 monoclonal antibody (Product # MA5-34906), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRDX6 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRDX6 monoclonal antibody (Product # MA5-34906), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRDX6 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRDX6 monoclonal antibody (Product # MA5-34906), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX6 in paraffin-embedded human placenta tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX6 in paraffin-embedded human placenta tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX6 in paraffin-embedded human liver tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX6 in paraffin-embedded human kidney tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRDX6 in paraffin-embedded human colon cancer tissue. Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PRDX6 in PC-3M cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with PRDX6 monoclonal antibody (Product # MA5-34906) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG.