PA5-24632

antibody from Invitrogen Antibodies

Targeting: PRDX6 1-Cys, aiPLA2, AOP2, KIAA0106, MGC46173, NSGPx, p29, PRX

Antibody data

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Validation data

Product number: PA5-24632 - Provider product page
Provider: Invitrogen Antibodies
Product name: PRDX6 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with bovine, non-human primate and rat based on sequence homology.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 400 µL
Concentration: 0.4 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PRDX6 protein structure - PA5-24632 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis using a PRDX6 polyclonal antibody (Product # PA5-24632) in 293 cell lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PRDX6 was achieved by transfecting MCF7 with PRDX6 specific siRNAs (Silencer® select Product # s18429). Western blot analysis (Fig. a) was performed using whole cell extracts from the PRDX6 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with PRDX6 Polyclonal Antibody (Product # PA5-24632, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PRDX6.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), HeLa (Lane 2), NTERA-2 cl.D1 (Lane 3), K-562 (Lane 4), Caco-2 (Lane 5), Hep G2 (Lane 6), LnCAP (Lane 7) and MDA-MB-231 (Lane 8). The blot was probed with Anti-PRDX6 Polyclonal Antibody (Product # PA5-24632, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 25 kDa band corresponding to PRDX6 was observed across the cell lines tested.