HPA024371

antibody from Atlas Antibodies

Targeting: SUPT3H SPT3, SPT3L

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA024371

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA024371, RRID:AB_1857660

Product name

Anti-SUPT3H

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SUPT3H, Gene description: suppressor of Ty 3 homolog (S. cerevisiae), Alternative Gene Names: SPT3, SPT3L, Validated applications: IHC, ICC, ChIP, Uniprot ID: O75486, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skin tissues using Anti-SUPT3H antibody. Corresponding SUPT3H RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-SUPT3H (HPA024371, Lot R11543) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.