Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29005 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T. Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (83%), Dog (93%), Pig (100%), Chicken (84%), Rhesus Monkey (93%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references miRNA‑183‑5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1.
Lin J, Shen J, Yue H, Cao Z
Oncology reports 2019 Dec;42(6):2371-2381
Oncology reports 2019 Dec;42(6):2371-2381
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TPM3 using 30 µg of A) 293T and B) A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a TPM3 polyclonal antibody (Product # PA5-29005) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Effect of miR-183-5p.1 on the expression of TPM2 and TPM3 in GC AGS cells. (A and B) miR-183-5p.1 mimics downregulated the expression of TPM2 and TPM3 as determined by western blotting. (C and D) miR-183-5p.1 inhibitors promoted the expression of TPM2 and TPM3 as detected by western blotting. Results are presented as the mean +- standard error of the mean (n=3). ***P
