PAB23825

antibody from Abnova Corporation

Targeting: KARS1 DFNB89, KARS, KARS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23825

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23825, RRID:AB_11121375

Product name

KARS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant KARS.

Antigen sequence

GLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQ
AKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMG
IDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATT
DTLEST


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with KARS polyclonal antibody (Cat # PAB23825) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with KARS polyclonal antibody (Cat # PAB23825) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (microtubules).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human hippocampus with KARS polyclonal antibody (Cat # PAB23825) shows strong cytoplasmic positivity in neuronal cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)