GTX118987

antibody from GeneTex

Targeting: PGR NR3C3, PR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

GTX118987

Provider

GeneTex

Proper citation

GeneTex Cat#GTX118987, RRID:AB_10618178

Product name

Progesterone Receptor (phospho Ser554) antibody

Antibody type

Polyclonal

Antigen

Other

Description

Purified by antigen-affinity chromatography.

Reactivity

Human, Mouse

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX118987 diluted at 1:10000

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Progesterone Receptor (phospho Ser554) antibody immunoprecipitates Progesterone Receptor (phospho Ser554) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 ?g of preimmune rabbit IgG B. Immunoprecipitation of Progesterone Receptor (phospho Ser554) protein by 2 ?g of Progesterone Receptor (phospho Ser554) antibody (GTX118987) 7.5% SDS-PAGE The immunoprecipitated Progesterone Receptor (phospho Ser554) protein was detected by Progesterone Receptor (phospho Ser554) antibody (GTX118987) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Validation comment

IP