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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Product number
- 37-8200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- 37-8200 detects Phospho-Progesterone Receptor in Human samples. 37-8200 has been successfully used in western blot, immunofluorescence and immunohistochemistry in human samples. Phospho-Progesterone Receptor (Ser 190) antibody detects both progesterone receptor A and B forms when phosphorylated at Ser26 and Ser190, respectively.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1154
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phosphorylated Progesterone Receptor (upper panel) and total Progesterone Receptor (lower panel) was performed by loading 20 µg of nuclear (N) or cytoplasmic (C) T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone (R5020) for 1 hour and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Phosphorylated Progesterone Receptor was detected using a Phospho-Progesterone Receptor (Ser190) mouse monoclonal antibody (upper panel, Product # 37-8200) at a dilution of 1:1000 and Total Progesterone Receptor was detected using a Progesterone Receptor mouse monoclonal antibody (lower panel, Product # MA1-12626) at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Superclonal goat anti-Mouse IgG-HRP secondary antibody (Product # A28177) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Progesterone Receptor in T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM). Showing specific immunolabeling of a ~90 kDa band corresponding to PR-A isoform and a ~120 kDa band corresponding to PR-B isoform of the Phospho-Progesterone Receptor (Ser190) monoclonal antibody (Product # 37-8200). The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Phospho-Progesterone Receptor pSer190 mouse monoclonal antibody (Product # 37-8200), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
