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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-34849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Progesterone Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Progesterone Receptor using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Progesterone Receptor polyclonal antibody (Product # PA5-34849) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Progesterone Receptor in paraformaldehyde-fixed A549 cells using a Progesterone Receptor polyclonal antibody (Product # PA5-34849) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Progesterone Receptor was performed in paraffin-embedded human breast cancer tissue using Progesterone Receptor Polyclonal Antibody (Product # PA5-34849) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. Representative data showing negative staining in PR-negative breast cancer (left) and nuclear staining in PR-positive breast cancer (right).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Progesterone receptor (Product # PA5-34849) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
