MA1-412

antibody from Invitrogen Antibodies

Targeting: PGR NR3C3, PR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Gel shift (Recommended by provider)

Antibody Data

Product number

MA1-412

Provider

Invitrogen Antibodies

Product name

Progesterone Receptor Monoclonal Antibody (alpha PR-22)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1-412 detects the A and B forms of progesterone receptor from chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor. MA1-412 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 78 kDa protein representing the A form and a 110 kDa protein representing the B form of PR. The proportion of receptor bound by this product is significantly greater than catalog number MA1-411 due to the binding of both the A and B form of PR. Protein A can effectively be used with this product in immunoprecipitation experiments. MA1-412 detects the A and B forms of progesterone receptor from human, chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor. The MA1-412 immunogen is progesterone receptor purified from chick oviduct cytosol. Reconstitute with PBS.

Reactivity

Human, Chicken/Avian

Host

Mouse

Isotype

IgG

Antibody clone number

alpha PR-22

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Synergistic Activation of Bovine Herpesvirus 1 Productive Infection and Viral Regulatory Promoters by the Progesterone Receptor and Krüppel-Like Transcription Factor 15.

El-Mayet FS, El-Habbaa AS, D'Offay J, Jones C
Journal of virology 2019 Jan 1;93(1)

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Uterine epithelial morphology and progesterone receptors in a mifepristone-treated viviparous lizard Pseudemoia entrecasteauxii (Squamata: Scincidae) during gestation.

Biazik JM, Parker SL, Murphy CR, Thompson MB
Journal of experimental zoology. Part B, Molecular and developmental evolution 2012 Mar;318(2):148-58

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Progesterone receptor deficient in chromatin binding has an altered cellular state.

Botos J, Xian W, Smith DF, Smith CL
The Journal of biological chemistry 2004 Apr 9;279(15):15231-9

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The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.

Felts SJ, Owen BA, Nguyen P, Trepel J, Donner DB, Toft DO
The Journal of biological chemistry 2000 Feb 4;275(5):3305-12

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The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.

Felts SJ, Owen BA, Nguyen P, Trepel J, Donner DB, Toft DO
The Journal of biological chemistry 2000 Feb 4;275(5):3305-12

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Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.

Prapapanich V, Chen S, Smith DF
Molecular and cellular biology 1998 Feb;18(2):944-52

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Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.

Prapapanich V, Chen S, Smith DF
Molecular and cellular biology 1998 Feb;18(2):944-52

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Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids.

Hu J, Toft DO, Seeger C
The EMBO journal 1997 Jan 2;16(1):59-68

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Molecular cloning of human p48, a transient component of progesterone receptor complexes and an Hsp70-binding protein.

Prapapanich V, Chen S, Nair SC, Rimerman RA, Smith DF
Molecular endocrinology (Baltimore, Md.) 1996 Apr;10(4):420-31

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A novel chaperone complex for steroid receptors involving heat shock proteins, immunophilins, and p23.

Johnson JL, Toft DO
The Journal of biological chemistry 1994 Oct 7;269(40):24989-93

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Characterization of a novel 23-kilodalton protein of unactive progesterone receptor complexes.

Johnson JL, Beito TG, Krco CJ, Toft DO
Molecular and cellular biology 1994 Mar;14(3):1956-63

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Chicken progesterone receptor is phosphorylated by a DNA-dependent protein kinase during in vitro transcription assays.

Weigel NL, Carter TH, Schrader WT, O'Malley BW
Molecular endocrinology (Baltimore, Md.) 1992 Jan;6(1):8-14

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Immunocytochemical study of progesterone receptors in hyperplastic and neoplastic endometrial tissues.

Bergeron C, Ferenczy A, Toft DO, Shyamala G
Cancer research 1988 Nov 1;48(21):6132-6

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Interaction of murine progesterone receptors with specific monoclonal antibodies to the avian progesterone receptor.

Schneider W, Toft DO, Sullivan WP, Shyamala G
Journal of steroid biochemistry 1988 Mar;29(3):297-306

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Progesterone Receptor was performed by loading 20 µg of T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone (R5020) for 1 hour and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Progesterone Receptor was detected using a Progesterone Receptor mouse monoclonal antibody, Product # MA1-412, at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Superclonal goat anti-Mouse IgG-HRP secondary antibody (Product # A28177) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with or without Progesterone Receptor mouse monoclonal antibody (Product # MA1-412), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Progesterone Receptor was done on T-47D cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Progesterone Receptor Mouse Monoclonal Antibody (MA1412, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.