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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB200-319 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-319, RRID:AB_10002265
- Product name
- Mouse Monoclonal Progesterone R/NR3C3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser190. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Progesterone Receptor [p Ser190] Antibody (1154) [NB200-319] - Whole cell T47D lysate prepared from cells that had been inubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
