MA5-12581

antibody from Invitrogen Antibodies

Targeting: PGR NR3C3, PR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12581

Provider

Invitrogen Antibodies

Product name

Progesterone Receptor Monoclonal Antibody (PgR636)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-12581 targets Progesterone Receptor in IHC (P) applications and shows reactivity with Human samples.

Antibody clone number

PgR636

Concentration

Conc. Not Determined

References

Submitted references

Hexokinase and phosphofructokinase activity and intracellular distribution correlate with aggressiveness and invasiveness of human breast carcinoma.

Coelho RG, Calaça IC, Celestrini DM, Correia-Carneiro AH, Costa MM, Zancan P, Sola-Penna M
Oncotarget 2015 Oct 6;6(30):29375-87

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Analysis of peptidyl-propyl-cis/trans isomerase 1 (PIN1) gene -842(G > C) and -667(T > C) polymorphic variants in relation to breast cancer risk and clinico-pathological parameters.

Naidu R, Har YC, Taib NA
Scandinavian journal of clinical and laboratory investigation 2011 Oct;71(6):500-6

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Expression of KAI1/CD82 in distant metastases from estrogen receptor-negative breast cancer.

Christgen M, Christgen H, Heil C, Krech T, Länger F, Kreipe H, Lehmann U
Cancer science 2009 Sep;100(9):1767-71

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Progesterone Receptor was performed by loading 20 µg of T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone (R5020) for 1 hour and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Progesterone Receptor was detected using a Progesterone Receptor mouse monoclonal antibody, Product # MA1-12581, at a dilution of 1:500 in blocking buffer overnight at 4°C on a rocking platform, followed by a Superclonal goat anti-Mouse IgG-HRP secondary antibody (Product # A28177) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Progesterone Receptor mouse monoclonal antibody (Product # MA5-12581), at a dilution of 1:100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human breast cancer stained with PR antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.