MA5-12658

antibody from Invitrogen Antibodies

Targeting: PGR NR3C3, PR

Antibody data

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Validation data

Product number: MA5-12658 - Provider product page
Provider: Invitrogen Antibodies
Product name: Progesterone Receptor Monoclonal Antibody (hPRa 7)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-12658 targets Progesterone Receptor in IF, IP, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
Antibody clone number: hPRa 7
Concentration: 0.2 mg/mL

PGR protein structure - MA5-12658 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with or without Progesterone Receptor mouse monoclonal antibody (Product # MA5-12658), at a concentration of 10 µg/mL for 1 hour at 37C, and then incubated with a Alexa Fluor 488 Superclonal goat anti-mouse IgG secondary antibody (Product # A28175) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Progesterone receptor was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Progesterone Receptor Monoclonal Antibody (hPRa 7) (Product # MA5-12658) at 1:50 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green).Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. Panel f shows the negative model, SK-BR-3, that does not express Progesterone receptor. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Pgr gene profiling in MF- and P4-treated transgenic Inhalpha/Tag TG mice and BLTK-1 cells proliferation and invasion. qPCR analysis of Pgr ( A ), Pgrmc1 ( B ), Pgrmc2 ( C ), Paqr7 (mPRalpha) ( D ), Paqr8 (mPRbeta) ( E ), and Paqr5 (mPRgamma) ( F ) expression in the non-, MF- and P4-treated tumors of Inhalpha/Tag TG mice. Each bar represents the mean +- SEM relative to Ppia. Immunohistochemical staining of PGR in the control ( G ), MF-treated ( H ) and P4-treated ( I ) tumors and of PGRMC1 in control ( J ), MF-treated ( K ) and P4-treated ( L ) LCTs of Inhalpha/Tag TG mice. Scale bar, 100 mum. Asterisks indicate significant differences between the control and treated groups (**, p < 0.01). C, control; Inhalpha/Tag TG mice; transgenic mice expressing the SV40 Taq oncogene under the inhibin alpha promoter; LCT, Leydig cell tumor; MF, mifepristone; P4, progesterone.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 PGR mRNA and protein are induced by the LH surge in granulosa cells. ( A ) PGR mRNA expression in eCG and hCG-primed granulosa cells at no eCG + hCG (unstim) and eCG + hCG 0-12 h post-hCG stimulation. RT-qPCR was performed on samples of 1-3 ovaries. N = 3 independent experiments, bars with different superscripts are significantly different, F 6,14 = 17.53, p < 0.0001 (one-way ANOVA). ( B ) Western blot of PGR in granulosa cells during ovulation (unstim or eCG + hCG 0-12 h post-hCG). Western blot was performed in biological triplicates, with 3-4 mice per time point in each replicate. One representative example of three highly similar results is shown. Full-length blot is available in Suppl. Data 3 .