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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-31314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Progesterone Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-31314 targets Progesterone Receptor in WB applications and shows reactivity with Human samples. The PA5-31314 immunogen is synthetic peptide corresponding to a region within amino acids 499 and 591 of Human Progesterone Receptor.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Progesterone Receptor using 30µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Progesterone Receptor polyclonal antibody (Product # PA5-31314) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Progesterone Receptor showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a Progesterone Receptor polyclonal antibody (Product # PA5-31314) diluted at 1:500. Blue: Hoechst 33342 staining.
