V3295

antibody from NSJ Bioreagents

Targeting: LGALS13 PLAC8, PP13

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3295

Provider

NSJ Bioreagents

Product name

Galectin 13 Antibody / LGALS13

Antibody type

Monoclonal

Description

This highly specific Galectin 13 antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PP13/1161

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the Galectin 13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human partial recombinant protein and 2) human K562 cell lysate with Galectin 13 antibody (clone PP13/1161). Expected molecular weight ~16 kDa (monomer) and ~32 kDa (dimer).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human partial recombinant protein and 2) human K562 cell lysate with Galectin 13 antibody (clone PP13/1161). Expected molecular weight ~16 kDa (monomer) and ~32 kDa (dimer).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human K562 cell lysate with Galectin 13 antibody (clone PP13/1161). Expected molecular weight ~16 kDa (monomer) and ~32 kDa (dimer).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human placental tissue lysate with Galectin 13 antibody (clone PP13/1161). Expected molecular weight ~16 kDa (monomer) and ~32 kDa (dimer).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human spleen with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human spleen with Galectin 13 antibody (clone PP13/1161). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Galectin 13 antibody (clone PP13/1161) as confirmation of integrity and purity.