Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB5110 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Reg3B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) Reg3A, rmReg3D, recombinant human Reg3D, recombinant rat (rr) Reg3B, or rrReg3G.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P35230
- Isotype
- IgG
- Antibody clone number
- 518630
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration.
Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model.
Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment.
Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice.
Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL, Larsen HL, Guiu J, Alves MRP, Rundsten CF, Johansen JV, Li Y, Madsen CD, Nakamura T, Watanabe M, Nielsen OH, Schweiger PJ, Piccolo S, Jensen KB
Cell stem cell 2018 Jan 4;22(1):35-49.e7
Cell stem cell 2018 Jan 4;22(1):35-49.e7
Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model.
Tsay TB, Yang MC, Chang WH, Chen PH, Chen LW
Journal of translational medicine 2018 Aug 13;16(1):225
Journal of translational medicine 2018 Aug 13;16(1):225
Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment.
Wu YY, Hsu CM, Chen PH, Fung CP, Chen LW
Infection and immunity 2014 May;82(5):1994-2005
Infection and immunity 2014 May;82(5):1994-2005
Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice.
Zechner D, Spitzner M, Bobrowski A, Knapp N, Kuhla A, Vollmar B
Diabetologia 2012 May;55(5):1526-34
Diabetologia 2012 May;55(5):1526-34
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Reg3B by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg3B Monoclonal Antibody (Catalog # MAB5110) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Reg3B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.