LS-C185925

antibody from LSBio

Targeting: NUP62 DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, p62, SNDI

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185925

Provider

LSBio

Product name

NUP62 Antibody (aa230-490) LS-C185925

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug whole cell lysate). A: Hep G2 , B: MOLT4 , C: Raji . 10% SDS PAGE. NUP62 antibody diluted at 1:5000

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: NIH-3T3, B: JC, C: BCL-1, D: C2C12. 7.5% SDS PAGE. NUP62 antibody diluted at 1:5000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: PC-12. 7.5% SDS PAGE. NUP62 antibody diluted at 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Nucleoporin p62 antibody detects NUP62 protein at Cytoplasm and Nucleus by immunofluorescent analysis. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. NUP62 protein stained by Nucleoporin p62 antibody diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded NCIN87 xenograft using nucleoporin p62 antibody at 1:500 dilution.