NB120-2736
antibody from Novus Biologicals
Targeting: NUP62
DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, p62, SNDI
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB120-2736 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-2736, RRID:AB_2224645
- Product name
- Armenian Hamster Monoclonal NUP62 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat tissues.
- Reactivity
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: NUP62 Antibody (RL31) [NB120-2736] - Analysis of Nucleoporin p62 was performed by loading whole cell lysates (20ug) of the indicated samples and 5ul of prestained protein ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. Nucleoporin p62 was detected at ~62kD in samples using a Nucleoporin p62 (RL31) monoclonal antibody at a dilution of 1:200 overnight at 4C on a rocking platform, followed by a goat anti-Armenian hamster IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature.