MA1-073

antibody from Invitrogen Antibodies

Targeting: NUP62 DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, p62, SNDI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-073

Provider

Invitrogen Antibodies

Product name

NUP62 Monoclonal Antibody (RL31)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-073 detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat, human and non-human primate tissues.

Antibody clone number

RL31

Concentration

0.5 mg/mL

References

Submitted references

Preferentially localized dynein and perinuclear dynactin associate with nuclear pore complex proteins to mediate genomic union during mammalian fertilization.

Payne C, Rawe V, Ramalho-Santos J, Simerly C, Schatten G
Journal of cell science 2003 Dec 1;116(Pt 23):4727-38

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Interaction of the transcription factor Sp1 with the nuclear pore protein p62 requires the C-terminal domain of p62.

Han I, Roos MD, Kudlow JE
Journal of cellular biochemistry 1998 Jan 1;68(1):50-61

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Interaction of the transcription factor Sp1 with the nuclear pore protein p62 requires the C-terminal domain of p62.

Han I, Roos MD, Kudlow JE
Journal of cellular biochemistry 1998 Jan 1;68(1):50-61

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Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.

Guan T, Müller S, Klier G, Panté N, Blevitt JM, Haner M, Paschal B, Aebi U, Gerace L
Molecular biology of the cell 1995 Nov;6(11):1591-603

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Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.

Guan T, Müller S, Klier G, Panté N, Blevitt JM, Haner M, Paschal B, Aebi U, Gerace L
Molecular biology of the cell 1995 Nov;6(11):1591-603

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A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.

Featherstone C, Darby MK, Gerace L
The Journal of cell biology 1988 Oct;107(4):1289-97

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nucleoporin p62 was performed by loading whole cell lysates (20 µg) of the indicated samples and 5 µL of Thermo Scientific PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. Nucleoporin p62 was detected at ~62 kD in samples using a Nucleoporin p62 (RL31) monoclonal antibody (Product # MA1-073) at a dilution of 1:200 overnight at 4C on a rocking platform, followed by a goat anti-armenian hamster IgG-HRP secondary antibody (Product # PA1-32045) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product # 34080).