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LearnGTX107973
antibody from GeneTex
Targeting: NUP62
DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, p62, SNDI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX107973 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107973, RRID:AB_1951041
- Product name
- Nucleoporin p62 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Inhibition of O-linked N-acetylglucosamine transferase activity in PC12 cells - A molecular mechanism of organophosphate flame retardants developmental neurotoxicity.
Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import.
Gu Y, Yang Y, Wan B, Li M, Guo LH
Biochemical pharmacology 2018 Jun;152:21-33
Biochemical pharmacology 2018 Jun;152:21-33
Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import.
Rodriguez-Bravo V, Pippa R, Song WM, Carceles-Cordon M, Dominguez-Andres A, Fujiwara N, Woo J, Koh AP, Ertel A, Lokareddy RK, Cuesta-Dominguez A, Kim RS, Rodriguez-Fernandez I, Li P, Gordon R, Hirschfield H, Prats JM, Reddy EP, Fatatis A, Petrylak DP, Gomella L, Kelly WK, Lowe SW, Knudsen KE, Galsky MD, Cingolani G, Lujambio A, Hoshida Y, Domingo-Domenech J
Cell 2018 Aug 23;174(5):1200-1215.e20
Cell 2018 Aug 23;174(5):1200-1215.e20
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 7.5% SDS PAGE GTX107973 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX107973 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate)A:Hep G2 (GTX27900)B:MOLT4 (GTX27912)C:Raji (GTX27908)10% SDS PAGEGTX107973 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nucleoporin p62 antibody detects Nucleoporin p62 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody (GTX107973) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody (GTX107973) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nucleoporin p62 antibody detects Nucleoporin p62 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody (GTX107973) diluted at 1:750.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Nucleoporin p62 antibody immunoprecipitates NUP62 protein in IP experiments.IP samples: 293T whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of NUP62 protein by 4 £gg Nucleoporin p62 antibody (GTX107973)7.5 % SDS-PAGEThe immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody (GTX107973) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 (GTX107973) antibody at 1:500 dilution.
