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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- HPA004890 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA004890, RRID:AB_1079935
- Product name
- Anti-SUGP1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SUGP1, Gene description: SURP and G patch domain containing 1, Alternative Gene Names: DKFZp434E2216, F23858, RBP, SF4, Validated applications: ICC, IHC, WB, Uniprot ID: Q8IWZ8, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Genetic alterations of SUGP1 mimic mutant-SF3B1 splice pattern in lung adenocarcinoma and other cancers
Alsafadi S, Dayot S, Tarin M, Houy A, Bellanger D, Cornella M, Wassef M, Waterfall J, Lehnert E, Roman-Roman S, Stern M, Popova T
Oncogene 2020;40(1):85-96
Oncogene 2020;40(1):85-96
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SUGP1 antibody. Remaining relative intensity is presented.
- Sample type
- Human
- Protocol
- Protocol
Enhanced validation
- Submitted by
- 55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein SUGP1 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where SUGP1 has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:161
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and liver tissues using HPA004890 antibody. Corresponding SUGP1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
