Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AF6429 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Thymosin beta 10 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Thymosin beta 10 in direct ELISAs. Detects human, mouse, and rat Thymosin beta 10 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Thymosin beta 4 and rhThymosin beta 16 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P63313
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Thymosin beta 10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Thymosin beta 10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6429) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 10 at approximately 6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Thymosin beta 10 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, NTera-2 human testicular embryonic carcinoma cell line, C6 rat glioma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Thymosin beta 10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6429) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thymosin beta 10 at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.