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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- LS-B10896 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ NUMA1 / NUMA Antibody (aa1-339) LS-B10896
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short term storage (1-2 weeks), store at 4°C. For long term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NuMA antibody [N1], N-term detects NUMA1 protein by Western blot analysis. A. 30 ug HeLa whole cell lysate/extract. B. 30 ug A375 whole cell lysate/extract. 5 % SDS-PAGE. NuMA antibody [N1], N-term dilution:1:1000
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NuMA antibody [N1], N-term detects NUMA1 protein by Western blot analysis. A. 30 ug HeLa whole cell lysate/extract. B. 30 ug A375 whole cell lysate/extract. 5 % SDS-PAGE. NuMA antibody [N1], N-term dilution:1:1000
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NuMA antibody [N1], N-term Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using NuMA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000. Scale bar = 10 m.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- NuMA antibody [N1], N-term Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using NuMA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000. Scale bar = 10 m.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NUMA1 / NUMA antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NUMA1 / NUMA antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
