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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX113510 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113510, RRID:AB_2037555
- Product name
- NuMA antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NuMA antibody [N1], N-term detects NUMA1 protein by Western blot analysis.A. 30 µg HeLa whole cell lysate/extractB. 30 µg A375 whole cell lysate/extract5 % SDS-PAGENuMA antibody [N1], N-term (GTX113510) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term (GTX113510) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with NuMA antibody [N1], N-term (GTX113510) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NuMA(GTX113510) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using NuMA (GTX113510) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NuMA antibody [N1], N-term detects NuMA protein at nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NuMA antibody [N1], N-term (GTX113510) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NuMA antibody [N1], N-term detects NuMA protein at nucleus in human esophageal cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal cancer. NuMA antibody [N1], N-term (GTX113510) diluted at 1:500.
