ABIN152988

antibody from antibodies-online

Targeting: NUMA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN152988

Provider

antibodies-online

Product name

anti-Nuclear Mitotic Apparatus Protein 1 (NUMA1) antibody

Antibody type

Polyclonal

Description

Unpurified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

0.1 mL

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Handling

Avoid freeze-thaw cycles

References

Submitted references

NuMA recruits dynein activity to microtubule minus-ends at mitosis.

Hueschen CL, Kenny SJ, Xu K, Dumont S
eLife 2017 Nov 29;6

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The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation.

Decarreau J, Wagenbach M, Lynch E, Halpern AR, Vaughan JC, Kollman J, Wordeman L
Nature cell biology 2017 Apr;19(4):384-390

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Transient PLK4 overexpression accelerates tumorigenesis in p53-deficient epidermis.

Serçin Ö, Larsimont JC, Karambelas AE, Marthiens V, Moers V, Boeckx B, Le Mercier M, Lambrechts D, Basto R, Blanpain C
Nature cell biology 2016 Jan;18(1):100-10

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The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation.

Mahale S, Kumar M, Sharma A, Babu A, Ranjan S, Sachidanandan C, Mylavarapu SVS
Scientific reports 2016 Dec 23;6(1):22

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Functional interplay between MYCN, NCYM, and OCT4 promotes aggressiveness of human neuroblastomas.

Kaneko Y, Suenaga Y, Islam SM, Matsumoto D, Nakamura Y, Ohira M, Yokoi S, Nakagawara A
Cancer science 2015 Jul;106(7):840-7

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Force on spindle microtubule minus ends moves chromosomes.

Elting MW, Hueschen CL, Udy DB, Dumont S
The Journal of cell biology 2014 Jul 21;206(2):245-56

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Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules.

Uchida C, Hattori T, Takahashi H, Yamamoto N, Kitagawa M, Taya Y
Genes to cells : devoted to molecular & cellular mechanisms 2014 Feb;19(2):89-96

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Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells.

Izumi H, Kaneko Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 30;109(44):18048-53

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A vascular niche and a VEGF-Nrp1 loop regulate the initiation and stemness of skin tumours.

Beck B, Driessens G, Goossens S, Youssef KK, Kuchnio A, Caauwe A, Sotiropoulou PA, Loges S, Lapouge G, Candi A, Mascre G, Drogat B, Dekoninck S, Haigh JJ, Carmeliet P, Blanpain C
Nature 2011 Oct 19;478(7369):399-403

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Role of epidermal primary cilia in the homeostasis of skin and hair follicles.

Croyle MJ, Lehman JM, O'Connor AK, Wong SY, Malarkey EB, Iribarne D, Dowdle WE, Schoeb TR, Verney ZM, Athar M, Michaud EJ, Reiter JF, Yoder BK
Development (Cambridge, England) 2011 May;138(9):1675-85

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Human papillomavirus E7 protein deregulates mitosis via an association with nuclear mitotic apparatus protein 1.

Nguyen CL, Münger K
Journal of virology 2009 Feb;83(4):1700-7

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Herpes simplex virus induces extensive modification and dynamic relocalisation of the nuclear mitotic apparatus (NuMA) protein in interphase cells.

Yamauchi Y, Kiriyama K, Kimura H, Nishiyama Y
Journal of cell science 2008 Jun 15;121(Pt 12):2087-96

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NuMA is required for the organization of microtubules into aster-like mitotic arrays.

Gaglio T, Saredi A, Compton DA
The Journal of cell biology 1995 Nov;131(3):693-708

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Image(s): Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

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Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC