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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- NB500-174 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-174, RRID:AB_10002562
- Product name
- Rabbit Polyclonal NuMA Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Drug Targeting the Actin Cytoskeleton Potentiates the Cytotoxicity of Low Dose Vincristine by Abrogating Actin-Mediated Repair of Spindle Defects.
Abnormal mitosis in reactive astrocytes.
Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity.
Microtubule End-Clustering Maintains a Steady-State Spindle Shape.
NuMA recruits dynein activity to microtubule minus-ends at mitosis.
Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.
An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.
The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation.
Transient PLK4 overexpression accelerates tumorigenesis in p53-deficient epidermis.
The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation.
Functional interplay between MYCN, NCYM, and OCT4 promotes aggressiveness of human neuroblastomas.
Force on spindle microtubule minus ends moves chromosomes.
Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules.
Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells.
A vascular niche and a VEGF-Nrp1 loop regulate the initiation and stemness of skin tumours.
Role of epidermal primary cilia in the homeostasis of skin and hair follicles.
Human papillomavirus E7 protein deregulates mitosis via an association with nuclear mitotic apparatus protein 1.
Herpes simplex virus induces extensive modification and dynamic relocalisation of the nuclear mitotic apparatus (NuMA) protein in interphase cells.
Wang Y, Stear JH, Swain A, Xu X, Bryce NS, Carnell M, Alieva IB, Dugina VB, Cripe TP, Stehn J, Hardeman EC, Gunning PW
Molecular cancer research : MCR 2020 Jul;18(7):1074-1087
Molecular cancer research : MCR 2020 Jul;18(7):1074-1087
Abnormal mitosis in reactive astrocytes.
Sosunov A, Wu X, McGovern R, Mikell C, McKhann GM 2nd, Goldman JE
Acta neuropathologica communications 2020 Apr 15;8(1):47
Acta neuropathologica communications 2020 Apr 15;8(1):47
Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity.
Gemble S, Simon A, Pennetier C, Dumont M, Hervé S, Meitinger F, Oegema K, Rodriguez R, Almouzni G, Fachinetti D, Basto R
Current biology : CB 2019 Sep 23;29(18):3072-3080.e5
Current biology : CB 2019 Sep 23;29(18):3072-3080.e5
Microtubule End-Clustering Maintains a Steady-State Spindle Shape.
Hueschen CL, Galstyan V, Amouzgar M, Phillips R, Dumont S
Current biology : CB 2019 Feb 18;29(4):700-708.e5
Current biology : CB 2019 Feb 18;29(4):700-708.e5
NuMA recruits dynein activity to microtubule minus-ends at mitosis.
Hueschen CL, Kenny SJ, Xu K, Dumont S
eLife 2017 Nov 29;6
eLife 2017 Nov 29;6
Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy.
Elting MW, Prakash M, Udy DB, Dumont S
Current biology : CB 2017 Jul 24;27(14):2112-2122.e5
Current biology : CB 2017 Jul 24;27(14):2112-2122.e5
An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.
di Pietro F, Valon L, Li Y, Goïame R, Genovesio A, Morin X
Current biology : CB 2017 Aug 21;27(16):2452-2464.e8
Current biology : CB 2017 Aug 21;27(16):2452-2464.e8
The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation.
Decarreau J, Wagenbach M, Lynch E, Halpern AR, Vaughan JC, Kollman J, Wordeman L
Nature cell biology 2017 Apr;19(4):384-390
Nature cell biology 2017 Apr;19(4):384-390
Transient PLK4 overexpression accelerates tumorigenesis in p53-deficient epidermis.
Serçin Ö, Larsimont JC, Karambelas AE, Marthiens V, Moers V, Boeckx B, Le Mercier M, Lambrechts D, Basto R, Blanpain C
Nature cell biology 2016 Jan;18(1):100-10
Nature cell biology 2016 Jan;18(1):100-10
The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation.
Mahale S, Kumar M, Sharma A, Babu A, Ranjan S, Sachidanandan C, Mylavarapu SVS
Scientific reports 2016 Dec 23;6(1):22
Scientific reports 2016 Dec 23;6(1):22
Functional interplay between MYCN, NCYM, and OCT4 promotes aggressiveness of human neuroblastomas.
Kaneko Y, Suenaga Y, Islam SM, Matsumoto D, Nakamura Y, Ohira M, Yokoi S, Nakagawara A
Cancer science 2015 Jul;106(7):840-7
Cancer science 2015 Jul;106(7):840-7
Force on spindle microtubule minus ends moves chromosomes.
Elting MW, Hueschen CL, Udy DB, Dumont S
The Journal of cell biology 2014 Jul 21;206(2):245-56
The Journal of cell biology 2014 Jul 21;206(2):245-56
Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules.
Uchida C, Hattori T, Takahashi H, Yamamoto N, Kitagawa M, Taya Y
Genes to cells : devoted to molecular & cellular mechanisms 2014 Feb;19(2):89-96
Genes to cells : devoted to molecular & cellular mechanisms 2014 Feb;19(2):89-96
Evidence of asymmetric cell division and centrosome inheritance in human neuroblastoma cells.
Izumi H, Kaneko Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 30;109(44):18048-53
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 30;109(44):18048-53
A vascular niche and a VEGF-Nrp1 loop regulate the initiation and stemness of skin tumours.
Beck B, Driessens G, Goossens S, Youssef KK, Kuchnio A, Caauwe A, Sotiropoulou PA, Loges S, Lapouge G, Candi A, Mascre G, Drogat B, Dekoninck S, Haigh JJ, Carmeliet P, Blanpain C
Nature 2011 Oct 19;478(7369):399-403
Nature 2011 Oct 19;478(7369):399-403
Role of epidermal primary cilia in the homeostasis of skin and hair follicles.
Croyle MJ, Lehman JM, O'Connor AK, Wong SY, Malarkey EB, Iribarne D, Dowdle WE, Schoeb TR, Verney ZM, Athar M, Michaud EJ, Reiter JF, Yoder BK
Development (Cambridge, England) 2011 May;138(9):1675-85
Development (Cambridge, England) 2011 May;138(9):1675-85
Human papillomavirus E7 protein deregulates mitosis via an association with nuclear mitotic apparatus protein 1.
Nguyen CL, Münger K
Journal of virology 2009 Feb;83(4):1700-7
Journal of virology 2009 Feb;83(4):1700-7
Herpes simplex virus induces extensive modification and dynamic relocalisation of the nuclear mitotic apparatus (NuMA) protein in interphase cells.
Yamauchi Y, Kiriyama K, Kimura H, Nishiyama Y
Journal of cell science 2008 Jun 15;121(Pt 12):2087-96
Journal of cell science 2008 Jun 15;121(Pt 12):2087-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NuMA Antibody [NB500-174] - Image shows a specific band for NuMA in 0.2 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NuMA Antibody [NB500-174] - Analysis of NuMA expression in Cos7 whole cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal brain using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells in the brain tissue depicted strong specific nuclear along with weak cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal breast using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The breast ductal/acinar epithelial cells, the myoepithelial cells and some cells in the surrounding connective tissue depicted a strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. The intra-lobular and the surrounding connective tissue also developed weak/potentially non-specific staining. 10X Magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal kidney using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells of various tubules/ducts and Bowman's capsule in the renal tissue depicted strong nuclear along with relatively weak cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal prostate using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The prostatic alveolar glandular epithelium as well as the surrounding fibromuscular stroma cells showed a strong nuclear immuno-reactivity for NuMA protein. Almost all the cells developed some cytoplasmic staining also. 10X Magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal skin using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Almost all the cells in the epidermal layer and the cells in the connective tissue of dermal layer depicted very strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal spleen using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Most of the cells developed a strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human placenta using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Strong nuclear with moderate cytoplasmic immuno-reactivity of NuMA was observed in syncytiotrophoblast, cytiotrophoblasts, Hofbauer cells and the endothelial cells of chorionic villi capillaries. 10X Magnification.
