NB300-535

antibody from Novus Biologicals

Targeting: KCNMB1 hslo-beta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NB300-535

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-535, RRID:AB_2131348

Product name

Rabbit Polyclonal Maxi Potassium channel beta Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Porcine, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100 ug

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration.

Echeverry S, Grismaldo A, Sánchez C, Sierra C, Henao JC, Granados ST, Sutachán JJ, Torres YP
Frontiers in physiology 2020;11:210

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Impaired function of coronary BK(Ca) channels in metabolic syndrome.

Borbouse L, Dick GM, Asano S, Bender SB, Dincer UD, Payne GA, Neeb ZP, Bratz IN, Sturek M, Tune JD
American journal of physiology. Heart and circulatory physiology 2009 Nov;297(5):H1629-37

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Interleukin-4 activates large-conductance, calcium-activated potassium (BKCa) channels in human airway smooth muscle cells.

Martin G, O'Connell RJ, Pietrzykowski AZ, Treistman SN, Ethier MF, Madison JM
Experimental physiology 2008 Jul;93(7):908-18

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Slo1 caveolin-binding motif, a mechanism of caveolin-1-Slo1 interaction regulating Slo1 surface expression.

Alioua A, Lu R, Kumar Y, Eghbali M, Kundu P, Toro L, Stefani E
The Journal of biological chemistry 2008 Feb 22;283(8):4808-17

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Somatic localization of a specific large-conductance calcium-activated potassium channel subtype controls compartmentalized ethanol sensitivity in the nucleus accumbens.

Martin G, Puig S, Pietrzykowski A, Zadek P, Emery P, Treistman S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 21;24(29):6563-72

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta (KCNMB1) was performed by loading 20ug of Movas whole cell lysates with and without Maxi K+ Beta (KCNMB1) siRNA and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ Beta rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta was performed by loading 20ug of Movas whole cell lysates and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane u and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.