681202
antibody from BioLegend
Targeting: NR5A2
B1F2, FTF, FTZ-F1, FTZ-F1beta, hB1F, hB1F-2, LRH-1, LRH1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 681202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681202, RRID:AB_2566455
- Product name
- Purified anti-NR5A2
- Antibody type
- Monoclonal
- Antigen
- Partial human NR5A2 recombinant protein (1-200 a.a.) expressed in E. coli.
- Description
- The nuclear receptor subfamily 5 group A member 2, also known as NR5A2, is a 54 kD nuclear receptor protein. NR5A2 is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. NR5A2 contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains. It also binds to DNA as a monomer. NR5A2 is known to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydrolase, which converts cholesterol to bile acids and also activates the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. NR5A2 has been suggested to regulate pancreas-specific gene expression and to play a role in embryonic development. There are at least 3 alternatively spliced isoforms of NR5A2: isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells HepG2 as well.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1D12B67
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysate from human HepG2 cells (lane 1) and mouse liver cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NR5A2 (clone 1D12B67) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
- Conjugate
- Unconjugated