606602
antibody from BioLegend
Targeting: NR5A2
B1F2, FTF, FTZ-F1, FTZ-F1beta, hB1F, hB1F-2, LRH-1, LRH1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 606602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#606602, RRID:AB_2154056
- Product name
- Purified anti-CPF
- Antibody type
- Polyclonal
- Antigen
- Peptide corresponding to amino acids 1-67 of human CPF, member 2, variant 2
- Description
- CPF, also known as nuclear receptor subfamily 5 group A member 2, orphan nuclear receptor NR5A2, HB1F, fetoprotein transcription factor, FTF, CYP7A promoter binding factor, and LRH1 is a 54 kD nuclear receptor protein. CPF is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. CPF contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains and binds to DNA as a monomer. CPF is known to regulate transcription of the liver enzyme cholesterol 7-alpha-hydrolase that converts cholesterol to bile acids and to activate the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. CPF interacts with multiprotein bridging actor 1 and the orphan nuclear receptor SHP and has been suggested to regulate pancreas-specific gene expression and play a role in embryonic development. There are at least 3 alternatively spliced isoforms of CPF; isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells such as HepG2. The Poly6066 recognizes human CPF (amino acids 1-67) and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-CPF antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated