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LearnPA5-28347
antibody from Invitrogen Antibodies
Targeting: NR5A2
B1F2, FTF, FTZ-F1, FTZ-F1beta, hB1F, hB1F-2, LRH-1, LRH1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-28347 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NR5A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse liver, Aspc1. Predicted reactivity: Mouse (88%), Rat (88%), Zebrafish (82%), Japanese Medaka (83%), Xenopus laevis (91%), Pig (96%), Chicken (96%), Sheep (95%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NR5A2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NR5A2 Polyclonal Antibody (Product # PA5-28347) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NR5A2 Polyclonal Antibody (Product # PA5-28347). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. NR5A2 Polyclonal Antibody (Product # PA5-28347) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NR5A2 in paraformaldehyde-fixed Human ESC cells using a NR5A2 polyclonal antibody (Product # PA5-28347) at a 1:400 dilution.
