PP-H2325-00

Product number: PP-H2325-00 - Provider product page
Provider: Perseus Proteomics Inc.
Product name: Anti human LRH-1 mouse monoclonal antibody
Antibody type: Monoclonal
Description: Ammonium sulfate-purified. Produced in hybridoma culture supernatant. Clone H2325 recognizes human LRH-1, widely applicable to advanced assays including ChIP, CUT&RUN, and CUT&Tag.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: H2325
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: 100 µL

NR5A2 protein structure - PP-H2325-00 shown in red.

Submitted references Feed-forward loops by NR5A2 ensure robust gene activation during pre-implantation development.

Kobayashi W, Ruangroengkulrith S, Arslantas EN, Mohanan A, Tachibana K
Development (Cambridge, England) 2026 Jan 1;153(1)

A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation.

Ren Y, Wang X, Liu H, Xu Y, Cheng R, Ren S, Li Z, Huo Y, Li B, Guan J, Li C, Deng H, Xu J
Nature cell biology 2025 Nov;27(11):1993-2006

Hepatic FXR-FGF4 is required for bile acid homeostasis via an FGFR4-LRH-1 signal node under cholestatic stress.

Song L, Hou Y, Xu D, Dai X, Luo J, Liu Y, Huang Z, Yang M, Chen J, Hu Y, Chen C, Tang Y, Rao Z, Ma J, Zheng M, Shi K, Cai C, Lu M, Tang R, Ma X, Xie C, Luo Y, Li X, Huang Z
Cell metabolism 2025 Jan 7;37(1):104-120.e9

Nr5a2 is dispensable for zygotic genome activation but essential for morula development.

Festuccia N, Vandormael-Pournin S, Chervova A, Geiselmann A, Langa-Vives F, Coux RX, Gonzalez I, Collet GG, Cohen-Tannoudji M, Navarro P
Science (New York, N.Y.) 2024 Oct 4;386(6717):eadg7325

Inhibiting NR5A2 targets stemness in pancreatic cancer by disrupting SOX2/MYC signaling and restoring chemosensitivity.

Zheng Q, Tang J, Aicher A, Bou Kheir T, Sabanovic B, Ananthanarayanan P, Reina C, Chen M, Gu JM, He B, Alcala S, Behrens D, Lawlo RT, Scarpa A, Hidalgo M, Sainz B Jr, Sancho P, Heeschen C
Journal of experimental & clinical cancer research : CR 2023 Nov 28;42(1):323

Nuclear receptor 5A2 regulation of Agrp underlies olanzapine-induced hyperphagia.

Zapata RC, Zhang D, Libster A, Porcu A, Montilla-Perez P, Nur A, Xu B, Zhang Z, Correa SM, Liu C, Telese F, Osborn O
Molecular psychiatry 2023 May;28(5):1857-1867

NR5A2 connects zygotic genome activation to the first lineage segregation in totipotent embryos.

Lai F, Li L, Hu X, Liu B, Zhu Z, Liu L, Fan Q, Tian H, Xu K, Lu X, Li Q, Feng K, Wang L, Lin Z, Deng H, Li J, Xie W
Cell research 2023 Dec;33(12):952-966

Asparagine protects pericentral hepatocytes during acute liver injury.

Sun Y, Demagny H, Faure A, Pontanari F, Jalil A, Bresciani N, Yildiz E, Korbelius M, Perino A, Schoonjans K
The Journal of clinical investigation 2023 Apr 3;133(7)

Reprogramming competence of OCT factors is determined by transactivation domains.

Kim KP, Wu Y, Yoon J, Adachi K, Wu G, Velychko S, MacCarthy CM, Shin B, Röpke A, Arauzo-Bravo MJ, Stehling M, Han DW, Gao Y, Kim J, Gao S, Schöler HR
Science advances 2020 Sep;6(36)

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Liu B, Xu Q, Wang Q, Feng S, Lai F, Wang P, Zheng F, Xiang Y, Wu J, Nie J, Qiu C, Xia W, Li L, Yu G, Lin Z, Xu K, Xiong Z, Kong F, Liu L, Huang C, Yu Y, Na J, Xie W
Nature 2020 Nov;587(7832):139-144

Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and Function.

Seacrist CD, Kuenze G, Hoffmann RM, Moeller BE, Burke JE, Meiler J, Blind RD
Structure (London, England : 1993) 2020 Jul 7;28(7):830-846.e9

Steroidogenic control of liver metabolism through a nuclear receptor-network.

Milona A, Massafra V, Vos H, Naik J, Artigas N, Paterson HAB, Bijsmans ITGW, Willemsen ECL, Ramos Pittol JM, Miguel-Aliaga I, Bosma P, Burgering BMT, Williamson C, Vernia S, Dhillo WS, van Mil SWC, Owen BM
Molecular metabolism 2019 Dec;30:221-229

LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival.

Bayrer JR, Wang H, Nattiv R, Suzawa M, Escusa HS, Fletterick RJ, Klein OD, Moore DD, Ingraham HA
Nature communications 2018 Oct 10;9(1):4055

Elevated copper impairs hepatic nuclear receptor function in Wilson's disease.

Wooton-Kee CR, Jain AK, Wagner M, Grusak MA, Finegold MJ, Lutsenko S, Moore DD
The Journal of clinical investigation 2015 Sep;125(9):3449-60

Correction: Nuclear receptor LRH-1/NR5A2 is required and targetable for liver endoplasmic reticulum stress resolution.

Mamrosh JL, Lee JM, Wagner M, Stambrook PJ, Whitby RJ, Sifers RN, Wu SP, Tsai MJ, DeMayo FJ, Moore DD
eLife 2015 Jul 24;4:e10084

Silencing LRH-1 in colon cancer cell lines impairs proliferation and alters gene expression programs.

Bayrer JR, Mukkamala S, Sablin EP, Webb P, Fletterick RJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 24;112(8):2467-72

Pancreatic-duodenal homeobox 1 regulates expression of liver receptor homolog 1 during pancreas development.

Annicotte JS, Fayard E, Swift GH, Selander L, Edlund H, Tanaka T, Kodama T, Schoonjans K, Auwerx J
Molecular and cellular biology 2003 Oct;23(19):6713-24

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Supportive validation

Submitted by: Perseus Proteomics Inc. (provider)
Main image
Experimental details: Antigen-coated ELISA using human LRH-1 as a coating antigen shows dose-dependent binding of anti-human LRH-1 mouse monoclonal antibody (H2325).