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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29981 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAPG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, NIH-3T3.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAPG using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CAPG polyclonal antibody (Product # PA5-29981) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CAPG Polyclonal Antibody (Product # PA5-29981). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. CAPG Polyclonal Antibody (Product # PA5-29981) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CAPG was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CAPG Polyclonal Antibody (Product # PA5-29981) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CAPG (Product # PA5-29981) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
