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LearnPA5-52146
antibody from Invitrogen Antibodies
Targeting: ARID1A
B120, BAF250, BAF250a, C10rf4, C1orf4, P270, SMARCF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-52146 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARID1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGLGNVAMGP RQHYPYGGPY DRVRTEPGIG PEGNMSTGAP QPNLMPSNPD SGMYSPSRYP PQQQQQQQQR HDSYGNQFST QGTPSGSPFP SQQTTMYQQQ QQNYK
- Concentration
- 0.17 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARID1A in NTERA-2 cells transfected with control siRNA, target specific siRNA probe #1, using a ARID1A Polyclonal Antibody (Product # PA5-52146). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ARID1A Polyclonal Antibody (Product # PA5-52146) and a 250 kDa band corresponding to ARID1A was observed in Mouse Spleen and Rat Spleen, but was not observed in Mouse Kidney and Rat Kidney which are known to have low expression levels of ARID1A (DOI: 10.1128/mcb.20.23.8879-8888.2000). Nuclear enriched extracts (30 µg lysate) of Mouse Spleen (Lane 1), Rat Spleen (Lane 2), Mouse Kidney (Lane 3) and Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). Some uncharacterized bands were observed between 45-55 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ARID1A in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an ARID1A Polyclonal Antibody (Product # PA5-52146).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ARID1A in human cell line U-251 MG using an ARID1A Polyclonal Antibody (Product # PA5-52146) shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARID1A in human colorectal cancer using an ARID1A Polyclonal Antibody (Product # PA5-52146) shows weak to moderate nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARID1A in human fallopian tube using an ARID1A Polyclonal Antibody (Product # PA5-52146) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARID1A in human duodenum using an ARID1A Polyclonal Antibody (Product # PA5-52146) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARID1A in human cerebral cortex using an ARID1A Polyclonal Antibody (Product # PA5-52146) shows moderate to strong nuclear positivity in neuronal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARID1A in human lymph node using an ARID1A Polyclonal Antibody (Product # PA5-52146) shows moderate nuclear positivity in lymphoid cells.
