PA5-24186

antibody from Invitrogen Antibodies

Targeting: FOLR1 FOLR, FRα

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24186

Provider

Invitrogen Antibodies

Product name

Folate Receptor alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A patch of positively charged residues regulates the efficacy of clinical DR5 antibodies in solid tumors.

Shivange G, Mondal T, Lyerly E, Bhatnagar S, Landen CN, Reddy S, Kim J, Doan B, Riddle P, Tushir-Singh J
Cell reports 2021 Nov 2;37(5):109953

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Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies.

Mondal T, Shivange GN, Tihagam RG, Lyerly E, Battista M, Talwar D, Mosavian R, Urbanek K, Rashid NS, Harrell JC, Bos PD, Stelow EB, Stack MS, Bhatnagar S, Tushir-Singh J
EMBO molecular medicine 2021 Mar 5;13(3):e12716

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CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.

Kleinmanns K, Fosse V, Davidson B, de Jalón EG, Tenstad O, Bjørge L, McCormack E
EBioMedicine 2020 Jun;56:102783

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Folate Receptor alpha in various lysates. Samples were incubated with Folate Receptor alpha polyclonal antibody (Product # PA5-24186) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: 293T/17 whole cell lysate; Lane 2: A549 whole cell lysate. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Folate Receptor alpha in lysate from Hela cell line. Samples were incubated with Folate Receptor alpha polyclonal antibody (Product # PA5-24186) using a dilution of 1:1,000 followed by goat anti-rabbit IgG H&L (HRP) at a dilution of 1:5,000. Lysate at 35 µg.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Folate Receptor alpha in paraffin-embedded Human kidney tissue. Samples were incubated with Folate Receptor alpha polyclonal antibody (Product # PA5-24186) using a dilution of 1:1,000 for 1 hours at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HeLa cells using a FOLR1 polyclonal antibody (Product # PA5-24186) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of (overlay histogram) of Folate Receptor alpha in Hela cells (green line). Samples were incubated with Folate Receptor alpha polyclonal antibody (Product # PA5-24186) using a dilution of 1:25 dilution for 60 min at 37°C followed by Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the primary antibody. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.