13473-1-AP

antibody from Proteintech Group

Targeting: SYVN1 DER3, HRD1

Provider product page for 13473-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [54]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 13473-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13473-1-AP, RRID:AB_2287023
Product name: HRD1/SYVN1 antibody
Antibody type: Polyclonal
Description: KD/KO validated HRD1/SYVN1 antibody (Cat. #13473-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SYVN1 protein structure - 13473-1-AP shown in red.

Submitted references HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress.

Wang L, Ren Z, Wu L, Zhang X, Wang M, Niu H, He X, Wang H, Chen Y, Shi G, Qian X
Molecular and cellular biochemistry 2024 Nov;479(11):3021-3036

Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death.

Weis D, Lin LL, Wang HH, Li ZJ, Kusikova K, Ciznar P, Wolf HM, Leiss-Piller A, Wang Z, Wei X, Weis S, Skalicka K, Hrckova G, Danisovic L, Soltysova A, Yang TT, Feichtinger RG, Mayr JA, Qi L
The Journal of clinical investigation 2024 Jan 16;134(2)

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders.

Wang HH, Lin LL, Li ZJ, Wei X, Askander O, Cappuccio G, Hashem MO, Hubert L, Munnich A, Alqahtani M, Pang Q, Burmeister M, Lu Y, Poirier K, Besmond C, Sun S, Brunetti-Pierri N, Alkuraya FS, Qi L
The Journal of clinical investigation 2024 Jan 16;134(2)

Cholesterol-induced HRD1 reduction accelerates vascular smooth muscle cell senescence via stimulation of endoplasmic reticulum stress-induced reactive oxygen species.

Wang L, Wang M, Niu H, Zhi Y, Li S, He X, Ren Z, Wen S, Wu L, Wen S, Zhang R, Wen Z, Yang J, Zhang X, Chen Y, Qian X, Shi G
Journal of molecular and cellular cardiology 2024 Feb;187:51-64

HRD1-induced TMEM2 ubiquitination promotes ER stress-mediated apoptosis through a non-canonical pathway in intestinal ischemia/reperfusion.

Zhao X, Liu D, Zhao Y, Wang Z, Wang Y, Chen Z, Ning S, Wang G, Meng L, Yao J, Tian X
Cell death & disease 2024 Feb 20;15(2):154

SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex.

Lin LL, Wang HH, Pederson B, Wei X, Torres M, Lu Y, Li ZJ, Liu X, Mao H, Wang H, Zhou LE, Zhao Z, Sun S, Qi L
Nature communications 2024 Feb 16;15(1):1440

Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis.

You HJ, Ma LH, Wang X, Wang YX, Zhang HY, Bao ES, Zhong YJ, Liu XY, Kong DL, Zheng KY, Kong FY, Tang RX
Cellular oncology (Dordrecht, Netherlands) 2024 Apr;47(2):639-655

Fufang Luohanguo Qingfei granules reduces influenza virus susceptibility via MAVS-dependent type I interferon antiviral signaling.

Lu YH, Wang M, Lin JQ, Wang MY, Zhou LY, He SH, Yi YT, Wei X, Huang QJ, Su ZH, Yang J, Guo HW, He RR, Luo Z
Journal of ethnopharmacology 2024 Apr 24;324:117780

Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression.

Guo Y, Shao J, Zhang R, Han M, Kong L, Liu Z, Li H, Wei D, Lu M, Zhang S, Zhang C, Wei H, Chen Z, Bian H
International journal of molecular sciences 2023 Sep 7;24(18)

Exogenous H(2)S initiating Nrf2/GPx4/GSH pathway through promoting Syvn1-Keap1 interaction in diabetic hearts.

Wang M, Tang J, Zhang S, Pang K, Zhao Y, Liu N, Huang J, Kang J, Dong S, Li H, Tian Z, Duan B, Lu F, Zhang W
Cell death discovery 2023 Oct 24;9(1):394

ER-associated degradation in cystinosis pathogenesis and the prospects of precision medicine.

Venkatarangan V, Zhang W, Yang X, Thoene J, Hahn SH, Li M
The Journal of clinical investigation 2023 Oct 2;133(19)

Involvement of Proteasomal and Endoplasmic Reticulum Stress in Neurodegeneration After Global Brain Ischemia.

Ziakova K, Kovalska M, Pilchova I, Dibdiakova K, Brodnanova M, Pokusa M, Kalenska D, Racay P
Molecular neurobiology 2023 Nov;60(11):6316-6329

UFMylation of HRD1 regulates endoplasmic reticulum homeostasis.

Luo H, Jiao QB, Shen CB, Gong WY, Yuan JH, Liu YY, Chen Z, Liu J, Xu XL, Cong YS, Zhang XW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Nov;37(11):e23221

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins.

Page KR, Nguyen VN, Pleiner T, Tomaleri GP, Wang ML, Guna A, Wang TY, Chou TF, Voorhees RM
bioRxiv : the preprint server for biology 2023 Nov 29;

SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool.

Ji Y, Luo Y, Wu Y, Sun Y, Zhao L, Xue Z, Sun M, Wei X, He Z, Wu SA, Lin LL, Lu Y, Chang L, Chen F, Chen S, Qian W, Xu X, Chen S, Pan D, Zhou Z, Xia S, Hu CA, Liang T, Qi L
Nature cell biology 2023 May;25(5):726-739

The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes.

Wu SA, Shen C, Wei X, Zhang X, Wang S, Chen X, Torres M, Lu Y, Lin LL, Wang HH, Hunter AH, Fang D, Sun S, Ivanova MI, Lin Y, Qi L
Nature communications 2023 May 30;14(1):3132

The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth.

Kambarev S, Borghesan E, Miller CN, Myeni S, Celli J
Infection and immunity 2023 May 16;91(5):e0013023

Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.

Zhang X, Young C, Liao XH, Refetoff S, Torres M, Tomer Y, Stefan-Lifshitz M, Zhang H, Larkin D, Fang D, Qi L, Arvan P
JCI insight 2023 Jun 22;8(12)

Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ER.

Yang J, Zhi Y, Wen S, Pan X, Wang H, He X, Lu Y, Zhu Y, Chen Y, Shi G
The Journal of nutritional biochemistry 2023 Jan;111:109178

Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin.

Thepsuwan P, Bhattacharya A, Song Z, Hippleheuser S, Feng S, Wei X, Das NK, Sierra M, Wei J, Fang D, Huang YM, Zhang K, Shah YM, Sun S
Proceedings of the National Academy of Sciences of the United States of America 2023 Jan 10;120(2):e2212644120

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Ganji R, Paulo JA, Xi Y, Kline I, Zhu J, Clemen CS, Weihl CC, Purdy JG, Gygi SP, Raman M
Nature communications 2023 Feb 6;14(1):638

Exogenous H(2) S promotes ubiquitin-mediated degradation of SREBP1 to alleviate diabetic cardiomyopathy via SYVN1 S-sulfhydration.

Zhang S, Wang M, Li H, Li Q, Liu N, Dong S, Zhao Y, Pang K, Huang J, Ren C, Wang Y, Tian Z, Lu F, Zhang W
Journal of cachexia, sarcopenia and muscle 2023 Dec;14(6):2719-2732

Role of SYVN1 in the control of airway remodeling in asthma protection by promoting SIRT2 ubiquitination and degradation.

Dai B, Liu S, Shen W, Chen L, Zhou Q, Han L, Zhang Q, Shan L
Biological research 2023 Dec 2;56(1):64

4-phenylbutyric acid re-trafficking hERG/G572R channel protein by modulating the endoplasmic reticulum stress-associated chaperones and endoplasmic reticulum-associated degradation gene.

Tang W, Cai D, Fu Y, Zheng Z, Huang X, Khouzam RN, Song Y, Lian J
Journal of thoracic disease 2023 Aug 31;15(8):4472-4485

Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism.

Abdon B, Liang Y, da Luz Scheffer D, Torres M, Shrestha N, Reinert RB, Lu Y, Pederson B, Bugarin-Lapuz A, Kersten S, Qi L
JCI insight 2023 Aug 3;8(17)

Pharmacological chaperones restore proteostasis of epilepsy-associated GABA(A) receptor variants.

Wang YJ, Seibert H, Ahn LY, Schaffer AE, Mu TW
bioRxiv : the preprint server for biology 2023 Apr 19;

Bombesin receptor-activated protein exacerbates cisplatin-induced AKI by regulating the degradation of SIRT2.

Peng L, Liu D, Liu H, Xia M, Wan L, Li M, Zhao J, Tang C, Chen G, Qu X, Dong Z, Liu H
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2022 Nov 23;37(12):2366-2385

Endoplasmic reticulum stress induces mitochondrial dysfunction but not mitochondrial unfolded protein response in SH-SY5Y cells.

Evinova A, Hatokova Z, Tatarkova Z, Brodnanova M, Dibdiakova K, Racay P
Molecular and cellular biochemistry 2022 Mar;477(3):965-975

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Sun Y, Fan Y, Wang Z, Li M, Su D, Liu Y, Liang X
Cellular and molecular life sciences : CMLS 2022 Mar 12;79(3):184

AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.

Shibuya K, Ebihara K, Ebihara C, Sawayama N, Isoda M, Yamamuro D, Takahashi M, Nagashima S, Ishibashi S
The Journal of biological chemistry 2022 Jun;298(6):101936

Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2.

Wei X, Zheng Z, Feng Z, Zheng L, Tao S, Zheng B, Huang B, Zhang X, Liu J, Chen Y, Zong W, Shan Z, Fan S, Chen J, Zhao F
EMBO molecular medicine 2022 Jul 7;14(7):e15373

SYVN1-MTR4-MAT2A Signaling Axis Regulates Methionine Metabolism in Glioma Cells.

Wang L, Hu B, Pan K, Chang J, Zhao X, Chen L, Lin H, Wang J, Zhou G, Xu W, Yuan J
Frontiers in cell and developmental biology 2021;9:633259

Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.

Dong JY, Xia KJ, Liang W, Liu LL, Yang F, Fang XS, Xiong YJ, Wang L, Zhou ZJ, Li CY, Zhang WD, Wang JY, Chen DP
Acta pharmacologica Sinica 2021 Sep;42(9):1461-1471

Hydrogen sulphide reduced the accumulation of lipid droplets in cardiac tissues of db/db mice via Hrd1 S-sulfhydration.

Sun Y, Zhang L, Lu B, Wen J, Wang M, Zhang S, Li Q, Shu F, Lu F, Liu N, Peng S, Zhao Y, Dong S, Lu F, Zhang W, Wang Y
Journal of cellular and molecular medicine 2021 Oct;25(19):9154-9167

PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.

Chen YJ, Knupp J, Arunagiri A, Haataja L, Arvan P, Tsai B
Nature communications 2021 Oct 13;12(1):5991

Preservation of endoplasmic reticulum (ER) Ca(2+) stores by deletion of inositol-1,4,5-trisphosphate receptor type 1 promotes ER retrotranslocation, proteostasis, and protein outer segment localization in cyclic nucleotide-gated channel-deficient cone photoreceptors.

Yang F, Ma H, Butler MR, Ding XQ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Jun;35(6):e21579

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Fan Y, Wang J, Jin W, Sun Y, Xu Y, Wang Y, Liang X, Su D
Molecular cancer 2021 Feb 2;20(1):25

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Li C, Chi H, Deng S, Wang H, Yao H, Wang Y, Chen D, Guo X, Fang JY, He F, Xu J
Journal for immunotherapy of cancer 2021 Aug;9(8)

SEL1L degradation intermediates stimulate cytosolic aggregation of polyglutamine-expanded protein.

Hattori T, Hanafusa K, Wada I, Hosokawa N
The FEBS journal 2021 Aug;288(15):4637-4654

STING regulates BCR signaling in normal and malignant B cells.

Tang CA, Lee AC, Chang S, Xu Q, Shao A, Lo Y, Spalek WT, Pinilla-Ibarz JA, Del Valle JR, Hu CA
Cellular & molecular immunology 2021 Apr;18(4):1016-1031

Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.

Yoshida S, Wei X, Zhang G, O'Connor CL, Torres M, Zhou Z, Lin L, Menon R, Xu X, Zheng W, Xiong Y, Otto E, Tang CA, Hua R, Verma R, Mori H, Zhang Y, Hu CA, Liu M, Garg P, Hodgin JB, Sun S, Bitzer M, Qi L
The Journal of clinical investigation 2021 Apr 1;131(7)

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5

Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.

Wang Y, Maeda Y, Liu YS, Takada Y, Ninomiya A, Hirata T, Fujita M, Murakami Y, Kinoshita T
Nature communications 2020 Feb 13;11(1):860

Exogenous H(2)S Induces Hrd1 S-sulfhydration and Prevents CD36 Translocation via VAMP3 Ubiquitylation in Diabetic Hearts.

Yu M, Du H, Wang B, Chen J, Lu F, Peng S, Sun Y, Liu N, Sun X, Shiyun D, Zhao Y, Wang Y, Zhao D, Lu F, Zhang W
Aging and disease 2020 Apr;11(2):286-300

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

Cunningham CN, Williams JM, Knupp J, Arunagiri A, Arvan P, Tsai B
Molecular cell 2019 Aug 8;75(3):442-456.e4

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.

van der Goot AT, Pearce MMP, Leto DE, Shaler TA, Kopito RR
Molecular cell 2018 May 3;70(3):516-530.e6

The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.

Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry 2018 Feb 16;293(7):2232-2246

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Luo H, Jiang M, Lian G, Liu Q, Shi M, Li TY, Song L, Ye J, He Y, Yao L, Zhang C, Lin ZZ, Zhang CS, Zhao TJ, Jia WP, Li P, Lin SY, Lin SC
Cell metabolism 2018 Apr 3;27(4):843-853.e6

Endoplasmic Reticulum Stress Induces HRD1 to Protect Alveolar Type II Epithelial Cells from Apoptosis Induced by Cigarette Smoke Extract.

Tan SX, Jiang DX, Hu RC, Dai AG, Gan GX, Fu DY, Kong CC, Chen YR, Wang LL, Li J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;43(4):1337-1345

Characterization of protein complexes of the endoplasmic reticulum-associated degradation E3 ubiquitin ligase Hrd1.

Hwang J, Walczak CP, Shaler TA, Olzmann JA, Zhang L, Elias JE, Kopito RR
The Journal of biological chemistry 2017 Jun 2;292(22):9104-9116

PDI reductase acts on Akita mutant proinsulin to initiate retrotranslocation along the Hrd1/Sel1L-p97 axis.

He K, Cunningham CN, Manickam N, Liu M, Arvan P, Tsai B
Molecular biology of the cell 2015 Oct 1;26(19):3413-23

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

Walczak CP, Ravindran MS, Inoue T, Tsai B
PLoS pathogens 2014 Mar;10(3):e1004007

A deubiquitinase negatively regulates retro-translocation of nonubiquitinated substrates.

Bernardi KM, Williams JM, Inoue T, Schultz A, Tsai B
Molecular biology of the cell 2013 Nov;24(22):3545-56

The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.

Williams JM, Inoue T, Banks L, Tsai B
Molecular biology of the cell 2013 Mar;24(6):785-95

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP(HRD1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The SYVN1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SYVN1. This antibody recognizes human,mouse,rat antigen. The SYVN1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 13473-1-AP.pdf

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