LS-C99633

antibody from LSBio

Targeting: SYVN1 DER3, HRD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C99633

Provider

LSBio

Product name

SYVN1 / HRD1 Antibody (aa586-617) LS-C99633

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot of SYVN1 (HRD1) Antibody in T47D cell line lysates (35 ug/lane). HRD1 (arrow) was detected using the purified antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

The anti-HRD1 antibody is used in Western blot to detect HRD1 in mouse kidney tissue lysate.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SYVN1 / HRD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.