NB100-2526

antibody from Novus Biologicals

Targeting: SYVN1 DER3, HRD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2526

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2526, RRID:AB_2271449

Product name

Rabbit Polyclonal HRD1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Hamster

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Membralin deficiency dysregulates astrocytic glutamate homeostasis leading to ALS-like impairment.

Jiang LL, Zhu B, Zhao Y, Li X, Liu T, Pina-Crespo J, Zhou L, Xu W, Rodriguez MJ, Yu H, Cleveland DW, Ravits J, Da Cruz S, Long T, Zhang D, Huang TY, Xu H
The Journal of clinical investigation 2019 May 21;129(8):3103-3120

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IRE1α RNase-dependent lipid homeostasis promotes survival in Myc-transformed cancers.

Xie H, Tang CH, Song JH, Mancuso A, Del Valle JR, Cao J, Xiang Y, Dang CV, Lan R, Sanchez DJ, Keith B, Hu CC, Simon MC
The Journal of clinical investigation 2018 Apr 2;128(4):1300-1316

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Characterization of protein complexes of the endoplasmic reticulum-associated degradation E3 ubiquitin ligase Hrd1.

Hwang J, Walczak CP, Shaler TA, Olzmann JA, Zhang L, Elias JE, Kopito RR
The Journal of biological chemistry 2017 Jun 2;292(22):9104-9116

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IRE1α is an endogenous substrate of endoplasmic-reticulum-associated degradation.

Sun S, Shi G, Sha H, Ji Y, Han X, Shu X, Ma H, Inoue T, Gao B, Kim H, Bu P, Guber RD, Shen X, Lee AH, Iwawaki T, Paton AW, Paton JC, Fang D, Tsai B, Yates JR 3rd, Wu H, Kersten S, Long Q, Duhamel GE, Simpson KW, Qi L
Nature cell biology 2015 Dec;17(12):1546-55

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Cytolethal distending toxins require components of the ER-associated degradation pathway for host cell entry.

Eshraghi A, Dixon SD, Tamilselvam B, Kim EJ, Gargi A, Kulik JC, Damoiseaux R, Blanke SR, Bradley KA
PLoS pathogens 2014 Jul;10(7):e1004295

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Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival.

Sun S, Shi G, Han X, Francisco AB, Ji Y, Mendonça N, Liu X, Locasale JW, Simpson KW, Duhamel GE, Kersten S, Yates JR 3rd, Long Q, Qi L
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 4;111(5):E582-91

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The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.

Koenig PA, Nicholls PK, Schmidt FI, Hagiwara M, Maruyama T, Frydman GH, Watson N, Page DC, Ploegh HL
The Journal of biological chemistry 2014 Dec 12;289(50):34490-502

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Derlin-2-deficient mice reveal an essential role for protein dislocation in chondrocytes.

Dougan SK, Hu CC, Paquet ME, Greenblatt MB, Kim J, Lilley BN, Watson N, Ploegh HL
Molecular and cellular biology 2011 Mar;31(6):1145-59

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Haploid insufficiency of suppressor enhancer Lin12 1-like (SEL1L) protein predisposes mice to high fat diet-induced hyperglycemia.

Francisco AB, Singh R, Sha H, Yan X, Qi L, Lei X, Long Q
The Journal of biological chemistry 2011 Jun 24;286(25):22275-82

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Glucocorticoid-induced Leucine zipper 1 stimulates the epithelial sodium channel by regulating serum- and glucocorticoid-induced kinase 1 stability and subcellular localization.

Soundararajan R, Wang J, Melters D, Pearce D
The Journal of biological chemistry 2010 Dec 17;285(51):39905-13

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HRD1 Antibody [NB100-2526] - Detection of HRD1 in an endogenous 293T cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HRD1 Antibody [NB100-2526] - Total protein from human HeLa, Hek293 and mouse 3T3 cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HRD1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HRD1 Antibody [NB100-2526] - Hrd1 is required for CDT intoxication. Co-immunoprecipitation of Derl2 and Hrd1. Derl2 was immunoprecipitated and samples were analyzed for Hrd1 by western blot. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004295) licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - FFPE tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2 ug/mL concentration (1:500 dilution). The primary antibody binding to SYVN1/HRD1 antigen in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This HRD1 antibody generated an expected cytoplasmic staining of HRD1 with stronger signal around periphery of the nucleus signifying the staining of the endoplasmic reticulum.