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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-85075 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Opsin 5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%). Positive Control: HeLaS3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Opsin 5 in A) HeLa S3 cells using Opsin 5 polyclonal antibody (Product # PA5-85075) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Opsin 5 in frozen adult mouse retina using Opsin 5 polyclonal antibody (Product # PA5-85075) at a dilution of 1:250. Sample was then incubated with beta Tubulin 3/ TUJ1 and DAPI secondary antibody at a dilution of 1:250.
