MA5-26855

antibody from Invitrogen Antibodies

Targeting: NR2C2 hTAK1, TAK1, TR2R1, TR4

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-26855

Provider

Invitrogen Antibodies

Product name

NR2C2 Monoclonal Antibody (OTI4B1)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI4B1

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

miR-616-5p Promotes Invasion and Migration of Bladder Cancer via Downregulating NR2C2 Expression.

Ren W, Hu J, Li H, Chen J, Ding J, Zu X, Fan B
Frontiers in oncology 2021;11:762946

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Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Ouyang X, Feng L, Yao L, Xiao Y, Hu X, Zhang G, Liu G, Wang Z
Molecular therapy. Nucleic acids 2021 Jun 4;24:755-767

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 TR4 promotes cell invasion/migration of PTC cells (A) TR4 expression level was examined in 20 pairs of primary PTC tumors and the paired lymph node metastatic PTC tumors using IHC staining strategy. Left, representative images of TR4 signal in PTC patients; top, image from primary PTC tumor; bottom, image from the paired PTC tumors metastasizing into lymph node; right, quantification of TR4 signal in PTC patients. Paired t test was used to make the statistical analysis. (B) TR4 expression level was significantly increased in nodal metastasis (N1) TC patients compared to non-metastasis (N0) TC patients. (C) OS analysis of TC patients classified by TR4 expression level. The blue line stands for low TR4 TPM; red line stands for high TR4 TPM. (D) Western blot assay was used to check the efficiency of TR4 knockdown in K-1 cells. Actin was internal control. (E) Knockdown of TR4 suppressed the cell invasion of K-1 cells. Left, representative images of invading K-1 cells; right, statistical analysis of invading K-1 cells. (F) Wound-healing assay showed that knockdown of TR4 inhibited cell migration of K-1 cells. Left, representative images of migrating K-1 cells; right, statistical analysis of migrating K-1 cells. (G) Western blot assay was used to check the efficiency of TR4 overexpression (oeTR4) in K-1 cells. GAPDH was loading control. (H) oeTR4 promoted cell invasion of K-1 cells. Left, representative images of invading K-1 cells; right, statistical analysis of invading

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 NR2C2 is negatively correlated with miR-616-5p and inhibits bladder cancer cell invasion and migration. (A) Western blot assay for screening the downstream target genes in T24 cells transduced with pLKO and miR-616-5p inhibitor and UMUC3 cells transduced with pLKO and oemiR-616-5p. (B) Correlation between miR-616 and NR2C2 expression in bladder cancer according to LinkedOmics database. (C) Western blot assay for NR2C2 expression in T24 cells transduced with pLKO and shNR2C2 and UMUC3 cells transduced with pWPI and oeNR2C2. (D) Chamber-transwell invasion and wound healing migration assays performed in T24 cells transduced with pLKO and shNR2C2 and UMUC3 cells transduced with pWPI and oeNR2C2. For (C) , quantification data are presented as mean +- SD, and significant differences are indicated by ** P < 0.01, and *** P < 0.001 compared to the controls.