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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00080347-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00080347-M01, RRID:AB_537880
- Product name
- Mouse Monoclonal COASY Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. COASY - Coenzyme A synthase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.Lane 1: COASY transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COASY Antibody (1H6) [H00080347-M01] - COASY monoclonal antibody (M01), clone 1H6 Analysis of COASY expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: COASY Antibody (1H6) [H00080347-M01] - Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.
